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Bones implants based on Fe and Mg
Košíček, Adam ; Zatloukal, Miroslav (referee) ; Sedlaříková, Marie (advisor)
This paper work deals with properties of metallic biomaterials in terms of their suitability for use as a temporary metal implants. The work focuses on biodegradable materials based on iron and suitable alloying elements, which would create a perfect implant. A part of this work describes procedure of creating biodegradable metallic samples with alloying element Magnesium and measuring the corrosion rates. There are a few theoretical chapters concerning permanent implants, function and composition of human bones, iron and magnesium in human body and biodegradable materials.
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Hodnocení vlivu složení krmné směsi na změnu biochemických parametrů krevní plazmy brojlerových kuřat
Šmalcová, Kateřina
The diploma thesis entitled "Evaluation of the influence of the composition of the feed mixture on the change in biochemical parameters of the blood plasma of broiler chickens" deals with the composition of the feed mixture and its effects on the internal environment and growth performance of broiler chickens. The aim was to develop a literature search on the topic of feeding broiler chickens and the biochemical parameters of blood plasma. Furthermore, check whether the supplemented feeding doses of milk thistle (Sylibum marianum L.) and the commercially produced preparations Presan - FY and Selko pH affect the indoor environment’s growth intensity and parameters. In the experiment, 3 control weighings and one final weighing were carried out, in which the actual increase was determined. Next, blood was taken into a tube with heparin, from which blood plasma was obtained for analysis of biochemical parameters. The data obtained from the analysis were statistically evaluated and statistically significant. A statistically significant difference was noted in the concentration of total bilirubin between groups O and K (p<0.05), but also between groups O and P (p<0.01), and there was also a statistically significant difference in the concentration of glucose between groups P and O (p<0.001) and also between P and K (p<0.01). The catalytic concentration of glutathione peroxidase was statistically significant between the O and P groups (p<0.05). There was a statistically significant difference in beta-hydroxybutyrate concentration between groups P and O (p<0.001), but also between groups P and K (p<0.05). Phosphorus concentration was statistically significant between K and O groups (p<0.01) and cholesterol concentration had a significant difference between O and K groups (p<0.001) and between O and P groups (p<0.05). There was a statistically significant difference in the concentration of non-esterified fatty acids between groups O and K (p<0.001), but also between groups O and P (p<0.05). Lactate dehydrogenase concentration was statistically significant between O and P groups (p<0.05) and creatine kinase level was statistically significant between O and K groups (p<0.01). It can be seen from the work that supplementation according to the methodology of the experiment influenced the growth intensity, the results of the measurements of the monitored environment of the animals and also the parameters of the inner environment of broiler chickens.
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Large Extracellular Vesicles in Cell Culture and Blood: Role in Prion Transmission and Detection by Flow Cytometry
Soukup, Jakub
Prions (PrP) are the main cause of neurodegenerative diseases such as Scrapie in sheep, bovine spongiform encephalopathy, chronic wasting disease in deer, and Creutzfeldt-Jakob disease in humans. Although the cellular PrP (PrPC ) is involved in many cellular processes, its precise function still needs to be discovered. The disease is caused by the accumulation of a pathological form of PrP (PrPTSE ), which is caused by direct contact of PrPTSE and PrPC . PrP is anchored in the membrane by GPI and can be transmitted by cell-to-cell contact, tunnelling nanotubes, or extracellular vesicles (EVs). EV factions are divided by different biogenesis into exosomes, microvesicles, and apoptotic bodies. PrPTSE was found in exosomes and microvesicles, but these fractions were never compared to each other. The first aim of the doctoral thesis is a comparison of PrP content, prion-converting activity and infectivity in these fractions on CAD5 and N2a-PK1 cellular models of infection. We isolated a fraction of large EVs (20,000× g) and small EVs (110,000× g) by centrifugation from a conditioned medium. We characterised EVs by cryo-electron microscopy and western blot with Alix, TSG-101, CD63, CD9, and HSP70 markers. The contamination from other cellular compartments was checked by calnexin. EV fractions differed...
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Large Extracellular Vesicles in Cell Culture and Blood: Role in Prion Transmission and Detection by Flow Cytometry
Soukup, Jakub ; Holada, Karel (advisor) ; Šebestová Janoušková, Olga (referee) ; Živný, Jan (referee)
Prions (PrP) are the main cause of neurodegenerative diseases such as Scrapie in sheep, bovine spongiform encephalopathy, chronic wasting disease in deer, and Creutzfeldt-Jakob disease in humans. Although the cellular PrP (PrPC ) is involved in many cellular processes, its precise function still needs to be discovered. The disease is caused by the accumulation of a pathological form of PrP (PrPTSE ), which is caused by direct contact of PrPTSE and PrPC . PrP is anchored in the membrane by GPI and can be transmitted by cell-to-cell contact, tunnelling nanotubes, or extracellular vesicles (EVs). EV factions are divided by different biogenesis into exosomes, microvesicles, and apoptotic bodies. PrPTSE was found in exosomes and microvesicles, but these fractions were never compared to each other. The first aim of the doctoral thesis is a comparison of PrP content, prion-converting activity and infectivity in these fractions on CAD5 and N2a-PK1 cellular models of infection. We isolated a fraction of large EVs (20,000× g) and small EVs (110,000× g) by centrifugation from a conditioned medium. We characterised EVs by cryo-electron microscopy and western blot with Alix, TSG-101, CD63, CD9, and HSP70 markers. The contamination from other cellular compartments was checked by calnexin. EV fractions differed...
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Donor eating habits and drinking schedule before plasmapheresis
Hostinová, Alena ; Englcová, Barbora (advisor) ; Strejčková, Vanda (referee)
(thesis): Background: This study explores the issue of eating habits and water intake of blood plasma donors. Objectives: The aim of this research was to map the blood plasma donors' awareness of well-balanced diet, evaluate their eating habits and water intake. Inquire donors' knowledge of plasmapheresis, their awareness of food suitable for replenishing proteins after the collection of blood plasma and iron in low levels of haemoglobin. Compare the differences in eating habits between men and women. Map the frequency of rejected plasma donors due to low haemoglobin, high level of leucocytes or chylous plasma. In the conclusion the respondents evaluate their own knowledge of the given problems. Methods: The research was conducted in a method of a standardised anonymous questionnaire. Overall data of 300 respondents attending a private plasma centre were processed while maintaining ethical principles. The research took place in 14 days, from 18th November 2015 until 2nd December 2015, so as to avoid a situation in which one client fills more than one questionnaire. The questionnaires were filled anonymously by clients waiting for their plasma to be taken or during the process in a donation chair. Results: 171 (57 %) of women and 129 (43 %) of men participated in the research. They were mostly young...
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Donor eating habits and drinking schedule before plasmapheresis
Hostinová, Alena ; Tuka, Vladimír (advisor) ; Indra, Tomáš (referee)
(thesis): Basis: This diploma thesis follows the bachelor thesis that concentrates on the eating habits and water intake of blood plasma donors before plasmapheresis. Goals: The research had four goals: 1) explore to what extent does providing of the educational material influence the awareness of blood plasma donors. 2) To map subjective state of health and lifestyle of plasma donors regarding their physical activity, healthy, regular, varied diet, smoking and sleep schedule. 3) To explore the knowledge of regime measures and risk behaviour before donating blood plasma. To evaluate donors' eating habits and water intake before plasmapheresis and reveal potential deficiencies. 4) Evaluate the knowledge of a given problem with those respondents who were provided with educational material in comparison with those respondents who got no educational material. Methods: An educational material was created regarding regime measures of blood plasma donors. This educational material served as the basis for standardized anonymous questionnaire. Maintaining the ethical principles and with the ethical committee's approval, overall data of 40 respondents frequenting Haematology Department and blood transfusion of the Military University Hospital Prague. The sample consisted of two groups of randomly selected...
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Determination of selected components in human urine with electrophoresis in short capillary.
Makrlíková, Anna
Capillary zone electrophoresis is frequently used in various analyses. In this diploma thesis a hydrodynamic sample introduction method controlled by pressure pulse has been proposed for short-capillary electrophoresis. The base electrolyte flushes sample from the loop of a six-way sampling valve and is carried to the injection end of the capillary. At the time when the sample zone reached the capillary, a short pressure impulse is generated in the electrolyte stream, which provides injection of the sample into the capillary. Then the electrolyte flow is stopped and the separation voltage is turned on. The amount of sample introduced to the capillary is controlled by the duration of the pressure pulse. This new sample introduction method was tested in the determination of ammonia, histidine, creatinine, uric acid and hippuric acid in human urine and for rapid screening of the contents of the inorganic ions in cerebrospinal fluid and blood plasma. The determination was performed in a capillary with an overall length of 10,5 cm and two base electrolytes was tested - 50 mM MES + 5 mM NaOH (pH 5,10) and 1 M acetic acid + 1,5 mM crown ether 18-crown-6 (pH 2,40). Using dual detection techniques contactless conductivity and UV spectrometric detection, anorganic and organic substances in the sample could...
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Determination of selected components in human urine with electrophoresis in short capillary.
Makrlíková, Anna
Capillary zone electrophoresis is frequently used in various analyses. In this diploma thesis a hydrodynamic sample introduction method controlled by pressure pulse has been proposed for short-capillary electrophoresis. The base electrolyte flushes sample from the loop of a six-way sampling valve and is carried to the injection end of the capillary. At the time when the sample zone reached the capillary, a short pressure impulse is generated in the electrolyte stream, which provides injection of the sample into the capillary. Then the electrolyte flow is stopped and the separation voltage is turned on. The amount of sample introduced to the capillary is controlled by the duration of the pressure pulse. This new sample introduction method was tested in the determination of ammonia, histidine, creatinine, uric acid and hippuric acid in human urine and for rapid screening of the contents of the inorganic ions in cerebrospinal fluid and blood plasma. The determination was performed in a capillary with an overall length of 10,5 cm and two base electrolytes was tested - 50 mM MES + 5 mM NaOH (pH 5,10) and 1 M acetic acid + 1,5 mM crown ether 18-crown-6 (pH 2,40). Using dual detection techniques contactless conductivity and UV spectrometric detection, anorganic and organic substances in the sample could...
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Involvement of donors of blood plasma to the donor's registry bone marrow
Míková, Iveta ; Kordulová, Pavla (advisor) ; Chvojková, Kateřina (referee)
The Bachelor thesis is focusing on the possibility to exploit the potential of regular blood plasma donors for inclusion into the register of bone marrow donors. Based on the assumption, that this is the almost identical population and that the criteria for blood plasma donating are similar to the criteria for bone marrow donation, plasma donors are tested periodically on the infectious diseases transmitted through blood, they have experience with the intravenous collection and plasma donors are largely complying with the age limit needed for bone marrow register. The theoretical part describes anatomy and physiology of blood plasma and bone marrow and current knowledge on the issue for donating of blood plasma and bone marrow. Describes types and indications of bone marrow transplantation, registers of blood plasma and bone marrow donors, the motivation for donating, reliefs and remunerations for donors. I have used for the making of this thesis processed research from Bibliographia medica tschechoslovacia in the National medical library, personal source of reference literature, professional consultation, own experiences and another Bachelor thesis. Methods: The Thesis was prepared based on quantitative research in the form of none standard anonymous questionnaire. The target group were the donors...
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